ffpe tissue sections  (Cell Signaling Technology Inc)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: GLUT1 expression, lymphocyte distribution and CD3 + T-cell metabolic subsets as predictive markers of response to immunotherapy in advanced melanoma

doi: 10.1186/s13046-025-03637-8

Figure Lengend Snippet: Associations between GLUT3 expression and immunotherapy response. A Representative multiplex image demonstrating expression of GLUT1 on SOX10 + tumor cells and CD31 + vessels, and GLUT3 expression on SOX10 + tumor cells and CD3 + T-cells. B 100% stacked bar plots illustrating GLUT1 + and GLUT3 + melanoma subsets as proportions of total SOX10 + melanoma cells. C 100% stacked bar plots illustrating GLUT1 + and GLUT3 + T-cell subsets as proportions of total CD3 + T-cells. D Bar plot showing no significant difference in the proportion of GLUT3 + melanoma between responders and non-responders. E Bar plots demonstrating significantly higher proportions of intratumoral GLUT3 + T-cells and GLUT3 + GLUT1 − T-cells in responders compared to non-responders. Error bars represent median ± 95% CI. * p < 0.05, ns – non-significant

Article Snippet: This included the sequential staining of FFPE tissue sections with CD31 (Cell Signaling, 1:1600) visualized with Opal570 TSA, GLUT1 (Cell Signaling, 1:4000) visualized with Opal620 TSA, GLUT3 (Cell Signaling, 1:4000) visualized with Opal520 TSA, SOX10 (Biocare Medical, 1:200) visualized with Opal690 TSA, CD3 (Cell Marque, 1:1000) visualized with Opal780 TSA and the DAPI counterstain.

Techniques: Expressing, Multiplex Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: GLUT1 expression, lymphocyte distribution and CD3 + T-cell metabolic subsets as predictive markers of response to immunotherapy in advanced melanoma

doi: 10.1186/s13046-025-03637-8

Figure Lengend Snippet: Associations between spatial locations of CD3 + T-cell subsets, CD31 + vessels and clinical outcomes. A Multiplex IF image and corresponding spatial plot showing locations of CD3 + T-cells (orange), GLUT3 + cells (green), CD31 + vessels (magenta) and SOX10 + melanoma cells (red). B Multiplex IF plot illustrating metabolic zonation pattern, with GLUT1 + and GLUT3 + melanoma located further away from CD31 + vessels. Bar plots showing all SOX10 + melanoma cells, GLUT1 + melanoma and GLUT3 + melanoma to be significantly further away from CD31 + vessels in tumors with metabolic zonation compared to those without metabolic zonation. C Bar plots showing CD3 + T-cells in closer proximity to GLUT3 + melanoma cells in responders compared to non-responders and significantly higher proportions of CD3 + T-cells within 20 µm of a GLUT3 + melanoma cell in responders. D Bar plots showing no significant differences in the average distances or spatial proximity of CD3 + , CD3 + GLUT1 + or CD3 + GLUT3 + T-cells to CD31 + vessels between responders and non-responders. Error bars represent median ± 95% CI. * p < 0.05, ** p < 0.01, ns – non-significant

Article Snippet: This included the sequential staining of FFPE tissue sections with CD31 (Cell Signaling, 1:1600) visualized with Opal570 TSA, GLUT1 (Cell Signaling, 1:4000) visualized with Opal620 TSA, GLUT3 (Cell Signaling, 1:4000) visualized with Opal520 TSA, SOX10 (Biocare Medical, 1:200) visualized with Opal690 TSA, CD3 (Cell Marque, 1:1000) visualized with Opal780 TSA and the DAPI counterstain.

Techniques: Multiplex Assay